Welcome to the Laboratory of Samrat Mukhopadhyay

Intrinsically Disordered Proteins, Liquid-Liquid Phase Separation, and Amyloid Formation

Overview

The overarching goal of our lab is to investigate a wide range of fascinating aspects of intrinsically disordered proteins/regions (IDPs/IDRs) that undergo liquid-liquid phase separation (LLPS) and amyloid formation. IDPs challenge the tenets of the traditional structure-function paradigm and exist as a rapidly interconverting conformational ensemble rather than a single well-defined structure. The conformational plasticity allows them to adopt different structures depending on their binding partners. Therefore, a single polypeptide sequence is capable of accomplishing a range of functions. It proposed that retaining disorder is an evolutionary strategy that allows complex functions within a compact genome of higher organisms. Additionally, the dysfunction of many IDPs is associated with a range of deadly diseases such as Alzheimer's and Parkinson's diseases and cancers. In our lab, we utilize a diverse array of tools and concepts from physical chemistry to chemical biology to biophysics and molecular biology to study the intriguing behavior of IDPs. Our efforts are aimed at delineating the fundamental molecular mechanism by which liquid-like condensates are formed and dissipated. We are also studying the liquid-to-solid phase transition into amyloid-like aggregates that are associated with deadly neurodegenerative diseases such as Alzheimer's, amyotrophic lateral sclerosis (ALS), and so forth.

We are interested in the following specific aspects of IDPs:

  • Conformational characteristics of IDPs and IDRs

  • Biomolecular condensation & liquid-liquid phase separation

  • Mechanism of amyloid formation

  • Functional prions and amyloids

  • Interactions of IDPs with lipid membrane

  • Hydration water in IDPs

  • Development and adaptation of new methodologies

For more details, see our Research page.

News and Events

  • Samrat was invited for a Nature podcast interview on our recent work on vibrational Raman spectroscopy of biomolecular condensates. Listen here.

  • Samrat recently spoke at a joint conference by The Brazilian Society of Biochemistry and Molecular Biology and The Brazilian Society of Biophysics in São Paulo, Brazil and delivered a seminar at the Federal University of Rio de Janeiro, Brazil.

  • Anamika wrote an invited blog post for Nature Portfolio on our recent work on vibrational Raman spectroscopy of biomolecular condensates. Click here to read.

  • Our latest paper on Single-droplet surface-enhanced Raman scattering is now published in Nature Communications. Click here

  • Our latest paper is now published in the Journal of Biological Chemistry. Click here.

  • Samrat's short interview for the Biophysical Society TV. Click here

  • Samrat recently spoke at the IDP Gordon Research Conference, EPFL Lausanne, EMBL Heidelberg and the University of Mainz.

  • Samrat spoke at the prestigious New and Notable session at the Biophysical Society meeting (San Francisco, February 2022). Click here


Research Funding

IISER Mohali

Centre for Protein Science, Design and Engineering (Centre of Excellence at IISER Mohali)